Spectrophotometric determination of the oxygen saturation of whole blood.

نویسندگان

  • M U TSAO
  • S S SETHNA
  • C H SLOAN
  • L J WYNGARDEN
چکیده

A new approach to the spectrophotometric determination of blood oxygen saturation has been made which is based on the absorption maximum of oxyhemoglobin and the isosbestic point of oxyhemoglobin and hemoglobin. The precision of this new method is comparable to that of the manometric procedure in which the Van Slyke-Neil1 apparatus is employed; there is also good correlation between results obtained with these procedures. The literature on the spectrophotometric method for the determination of blood oxygen has been reviewed by Hickam and Frayser (1). Since that publication the noteworthy advance has been made by Nahas who utilized the isosbestic point of hemoglobin for the determination of oxygen saturation of blood and designed a I.ucite cuvette of short light path (2). In an investigation in which the simultaneous determination of oxygen tension and saturation was to be made we found the time limitation required for the procedure for oxygen saturation as described by Nahas unfeasible. Furthermore, the precision of that method was not great enough for our purpose.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 217 1  شماره 

صفحات  -

تاریخ انتشار 1955